{"id":9319,"date":"2020-07-14T11:37:13","date_gmt":"2020-07-14T03:37:13","guid":{"rendered":"http:\/\/web.csh.org.tw\/web\/csh\/?p=9319"},"modified":"2024-11-13T11:36:19","modified_gmt":"2024-11-13T03:36:19","slug":"%e6%87%89%e7%94%a8%e4%b8%89%e6%b0%a7%e5%8c%96%e4%ba%8c%e7%a0%b7%e7%b6%93%e7%94%b1%e7%b4%b0%e8%83%9e%e6%ad%bb%e4%ba%a1%e7%8d%a8%e7%89%b9%e7%9a%84%e8%b7%af%e5%be%91%e6%94%b9%e5%96%84abt-737%e5%9c%a8","status":"publish","type":"post","link":"https:\/\/web.csh.org.tw\/web\/csh\/?p=9319","title":{"rendered":"\u61c9\u7528\u4e09\u6c27\u5316\u4e8c\u7837\u7d93\u7531\u7d30\u80de\u6b7b\u4ea1\u7368\u7279\u7684\u8def\u5f91\u6539\u5584ABT-737\u5728\u5b50\u5bae\u9838\u764c\u7d30\u80de\u7684\u6a19\u9776\u6cbb\u7642"},"content":{"rendered":"<p><strong>1.<\/strong><strong>\u7814\u7a76\u7d50\u679c\uff1a<\/strong><\/p>\n<p>ABT-737,4-[4-[(4&#8242;-chloro[1,1&#8242;-biphenyl]-2-yl)methyl]-1-piperazinyl]-N-[[4-[[(1R)-3-(di methylamino)-1-[(phenylthio)methyl]propyl]amino]-3-nitrophenyl] sulfonyl]-benzamide\u7531MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide)\u8a66\u9a57\u767c\u73fe\uff0c\u7576\u52a0\u4e0aarsenic trioxide (As<sub>2<\/sub>O<sub>3<\/sub>\uff1b\u4e09\u6c27\u5316\u4e8c\u7837)\uff0c\u53ef\u4ee5\u5728\u5b50\u5bae\u9838\u764c\u7d30\u80de\u8a98\u5c0e\u51fa\u4e00\u5354\u540c\u7684\u6bba\u6b7b\u764c\u7d30\u80de\u6548\u679c(synergistic effect on cell death of uterine cervical cancer)\u3002\u7d93\u7531flow cytometry\u7d30\u80de\u6d41\u5f0f\u5100\u4e4b\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u767c\u73feABT-737\u548cAs<sub>2<\/sub>O<sub>3<\/sub>\u5c0d\u5b50\u5bae\u9838\u764c\u7d30\u80de\u7684\u7d30\u80de\u6bd2\u6bba\u4f5c\u7528\uff0c\u5177\u6709\u5354\u540c\u6548\u679c\uff1b\u5305\u62ec\u7d30\u80de\u51cb\u4ea1\u6bd4\u7387(apoptotic percentage)\uff0c\u6703\u5448\u73fe\u6709\u610f\u7fa9\u7684\u4e0a\u5347\uff0c\u4e5f\u53ef\u767c\u73fe\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d(mitochondrial membrane potential)\u4e4b\u6d88\u5931\u3002\u7d93\u7531\u897f\u65b9\u9ede\u58a8\u6cd5( Western\u00a0blot assay)\u767c\u73fe\uff0c\u5169\u8005\u4f75\u7528\u53ef\u964d\u4f4ecyclin-dependent kinase 6 (CDK6)\u548cthymidylate synthetase (TS)\u7684\u542b\u91cf\u3002\u5169\u8005\u4f75\u7528\uff0c\u53ef\u6e2c\u5230\u589e\u52a0\u7d30\u80de\u51cb\u4ea1\u76f8\u95dc\u86cb\u767dcaspacse-7\u8868\u73fe\uff0c\u6e1b\u5c11\u6297\u51cb\u4ea1\u86cb\u767d(anti-apoptosis protein) myeloid\u00a0cell\u00a0leukemia\u00a01\u8868\u73fe\uff0c\u4e26\u00a0\u6291\u5236adenine nuclotide translocase\u86cb\u767d\u8868\u73fe\u3002<\/p>\n<p><strong>2.\u7814\u7a76\u8ca2\u737b\u8207\u81e8\u5e8a\u61c9\u7528\uff1a<\/strong><\/p>\n<p><strong>(1) <\/strong><strong>\u7814\u7a76\u8ca2\u737b<\/strong><\/p>\n<p>ABT-737\u52a0As<sub>2<\/sub>O<sub>3<\/sub>\uff0c\u53ef\u4ee5\u900f\u904e\u7368\u7279\u7684\u7d30\u80de\u6b7b\u4ea1\u8def\u5f91\uff0c\u5305\u62ec\u900f\u904e\u589e\u52a0caspacse-7\u548c\u964d\u4f4e\u7c92\u7dda\u9ad4\u819c\u96fb\u4f4d\u4fc3\u9032\u7d30\u80de\u51cb\u4ea1\uff1b\u6291\u5236TS\u5408\u6210\uff0c\u5f71\u97ffDNA\u5408\u6210\uff1b\u964d\u4f4eCDK6\u8868\u73fe\uff0c\u5f71\u97ffcell cycle\uff0c\u589e\u5f37\u5b50\u5bae\u9838\u764c\u7d30\u80de\u6b7b\u4ea1\u3002<\/p>\n<p><strong>(2) <\/strong><strong>\u81e8\u5e8a\u61c9\u7528<\/strong><\/p>\n<p>\u5b50\u5bae\u9838\u764c\u75c5\u4eba\u5728\u63a5\u53d7\u6cbb\u7642\u6642\uff0c\u4f75\u7528ABT-737\u548cAs<sub>2<\/sub>O<sub>3<\/sub>\uff0c\u5177\u6709\u589e\u52a0\u6bba\u6b7b\u764c\u7d30\u80de\u4e4b\u8f14\u52a9\u6027\u6cbb\u7642\u7684\u6f5b\u529b\u3002<!--more--><\/p>\n<p><strong><a href=\"https:\/\/web.csh.org.tw\/web\/csh\/wp-content\/uploads\/2020\/07\/2021-3.jpg\"><img 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[&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-9319","post","type-post","status-publish","format-standard","hentry","category-4"],"_links":{"self":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/9319"}],"collection":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9319"}],"version-history":[{"count":5,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/9319\/revisions"}],"predecessor-version":[{"id":24569,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/9319\/revisions\/24569"}],"wp:attachment":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9319"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9319"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9319"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}