{"id":4858,"date":"2018-02-12T09:11:11","date_gmt":"2018-02-12T01:11:11","guid":{"rendered":"http:\/\/web.csh.org.tw\/web\/csh\/?p=4858"},"modified":"2024-11-13T11:39:29","modified_gmt":"2024-11-13T03:39:29","slug":"%e8%a4%aa%e9%bb%91%e6%bf%80%e7%b4%a0%e6%8a%91%e5%88%b6%e4%ba%ba%e9%a1%9e%e9%aa%a8%e7%99%8c%e7%b4%b0%e8%83%9eccl24%e8%a1%a8%e7%8f%be%e5%8f%8a%e4%be%b5%e8%a5%b2%e6%a9%9f%e5%88%b6%e6%8e%a2%e8%a8%8e-2","status":"publish","type":"post","link":"https:\/\/web.csh.org.tw\/web\/csh\/?p=4858","title":{"rendered":"\u892a\u9ed1\u6fc0\u7d20\u6291\u5236\u4eba\u985e\u9aa8\u764c\u7d30\u80deCCL24\u8868\u73fe\u53ca\u4fb5\u8972\u6a5f\u5236\u63a2\u8a0e"},"content":{"rendered":"<p><strong>1.<\/strong><strong>\u7814\u7a76\u7d50\u679c\uff1a<\/strong><\/p>\n<p>\u672c\u8ad6\u6587\u4ee5\u7d30\u80de\u6bd2\u6027\u5be6\u9a57\u89c0\u5bdf\u5230\u892a\u9ed1\u6fc0\u7d20\u5728\u9aa8\u8089\u7624\u7d30\u80de\u682a\uff08U2OS\u548cHOS\uff09\u4e2d\u4e26\u7121\u7d30\u80de\u6bd2\u6027\u3002\u63a5\u8457\u4ee5Wound healing Assay\u548cboyden chamber Assay\u767c\u73fe\u892a\u9ed1\u6fc0\u7d20\u78ba\u5be6\u80fd\u6291\u5236\u9aa8\u8089\u7624\u7d30\u80de\u7684\u722c\u884c\u548c\u4fb5\u8972\u80fd\u529b\u3002\u5229\u7528 RNA-Sequencing (RNA-seq) \u627e\u51fa\u892a\u9ed1\u6fc0\u7d20\u53ef\u80fd\u5f71\u97ff\u8f49\u79fb\u4e4b\u76f8\u95dc\u57fa\u56e0CCL24\uff0c\u63a5\u8457\u4ee5Reverse transcription-polymerase chain reaction (RT-PCR)\u3001real-time PCR\u3001enzyme-linked immunosorbent assay (ELISA) \u767c\u73fe\u892a\u9ed1\u6fc0\u7d20\u80fd\u6709\u6548\u6291\u5236CCL24 mRNA\u53ca\u5176\u5206\u6ccc\u91cf\u3002\u5229\u7528Western blot\u767c\u73fe\u4eba\u985e\u9aa8\u8089\u7624\u7d30\u80deU2OS\u5728\u8655\u7406\u892a\u9ed1\u6fc0\u7d20\u5f8c\uff0c\u662f\u900f\u904e\u6291\u5236\u78f7\u9178\u5316JNK1\/2\u86cb\u767d\u8868\u73fe\u4f86\u6291\u5236\u7d30\u80de\u7684\u8f49\u79fb\u3002<\/p>\n<p>2.<strong>\u7814\u7a76\u8ca2\u737b\u8207\u81e8\u5e8a\u61c9\u7528\uff1a<\/strong><\/p>\n<p>\u7e3d\u7d50\u4ee5\u4e0a\u7d50\u679c\uff0c\u6211\u5011\u767c\u73fe\u5728\u9aa8\u8089\u7624\u7d30\u80de\u4e2d\uff0c\u892a\u9ed1\u6fc0\u7d20\u80fd\u6291\u5236\u8a0a\u606f\u50b3\u905e\u8def\u5f91\u4e2d\u7684JNK1\/2\u8a0a\u606f\u50b3\u905e\u8def\u5f91\u4f86\u964d\u4f4eCCL24\u7684\u5206\u6ccc\uff0c\u4f86\u9054\u5230\u6291\u5236\u9aa8\u8089\u7624\u7d30\u80de\u8f49\u79fb\u548c\u4fb5\u8972\u7684\u80fd\u529b\u3002\u56e0\u6b64\uff0c\u892a\u9ed1\u6fc0\u7d20\u53ef\u80fd\u53ef\u4ee5\u7528\u4f86\u9810\u9632\u9aa8\u8089\u7624\u7684\u8f49\u79fb\uff0c\u4e26\u671f\u671b\u80fd\u61c9\u7528\u5728\u672a\u4f86\u764c\u75c7\u7684\u6cbb\u7642\u4e0a\u3002<\/p>\n<p><!--more--><strong><a href=\"https:\/\/web.csh.org.tw\/web\/csh\/wp-content\/uploads\/2018\/02\/2018-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-24480\" src=\"https:\/\/web.csh.org.tw\/web\/csh\/wp-content\/uploads\/2018\/02\/2018-1.jpg\" alt=\"\" width=\"720\" height=\"960\" \/><\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>1.\u7814\u7a76\u7d50\u679c\uff1a \u672c\u8ad6\u6587\u4ee5\u7d30\u80de\u6bd2\u6027\u5be6\u9a57\u89c0\u5bdf\u5230\u892a\u9ed1\u6fc0\u7d20\u5728\u9aa8\u8089\u7624\u7d30\u80de\u682a\uff08U2OS\u548cHOS\uff09\u4e2d\u4e26\u7121\u7d30\u80de\u6bd2\u6027\u3002\u63a5\u8457\u4ee5Wo [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-4858","post","type-post","status-publish","format-standard","hentry","category-4"],"_links":{"self":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/4858"}],"collection":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4858"}],"version-history":[{"count":4,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/4858\/revisions"}],"predecessor-version":[{"id":24620,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/4858\/revisions\/24620"}],"wp:attachment":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4858"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4858"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4858"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}