{"id":21614,"date":"2023-01-05T17:28:17","date_gmt":"2023-01-05T09:28:17","guid":{"rendered":"http:\/\/web.csh.org.tw\/web\/csh\/?p=21614"},"modified":"2024-11-13T11:30:03","modified_gmt":"2024-11-13T03:30:03","slug":"cytotoxicity-and-apoptoticmechanismof-2-hydroxyethyl-methacrylate-via-genotoxicity-and-the-mitochondrial-dependent-intrinsic-caspase-pathway-and-intracellular-reactive-oxygen-species-accumulation-in","status":"publish","type":"post","link":"https:\/\/web.csh.org.tw\/web\/csh\/?p=21614","title":{"rendered":"Cytotoxicity and ApoptoticMechanismof 2-Hydroxyethyl Methacrylate via Genotoxicity and the Mitochondrial-Dependent Intrinsic Caspase Pathway and Intracellular Reactive Oxygen Species Accumulation in Macrophages"},"content":{"rendered":"<p><strong>1.<\/strong><strong>\u7814\u7a76\u7d50\u679c\uff1a<\/strong><\/p>\n<p>The results showed that HEMA induced cell toxicity in RAW264.7 macrophages depending on the concentration. A higher HEMA concentration was associated with a higher level of apoptosis and genotoxicity.Moreover, HEMA induced a concentration-dependent increase in mitochondrial dysfunction and the intrinsic caspase pathway, including the activation of caspase-3 and caspase-9. HEMA was also found to upregulate intracellular reactive oxygen species generation and to decrease the activity of antioxidant enzymes, including superoxide dismutase and catalase.<\/p>\n<p><strong>2.\u7814\u7a76\u8ca2\u737b\u8207\u81e8\u5e8a\u61c9\u7528\uff1a<\/strong><\/p>\n<p>Taken together, the mitochondrial-dependent\u00a0intrinsic caspase pathway and intracellular reactive oxygen species accumulation were found to mediate HEMA-induced genotoxicity and apoptosis, leading to cytotoxicity in RAW264.7 macrophages<\/p>\n<p><!--more--><\/p>\n<p><strong><a href=\"https:\/\/web.csh.org.tw\/web\/csh\/wp-content\/uploads\/2023\/01\/2022-1-6.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-25882\" src=\"https:\/\/web.csh.org.tw\/web\/csh\/wp-content\/uploads\/2023\/01\/2022-1-6.jpg\" alt=\"\" width=\"720\" height=\"960\" \/><\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>1.\u7814\u7a76\u7d50\u679c\uff1a The results showed that HEMA induced cell toxic [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-21614","post","type-post","status-publish","format-standard","hentry","category-4"],"_links":{"self":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/21614"}],"collection":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=21614"}],"version-history":[{"count":2,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/21614\/revisions"}],"predecessor-version":[{"id":25883,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=\/wp\/v2\/posts\/21614\/revisions\/25883"}],"wp:attachment":[{"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=21614"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=21614"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/web.csh.org.tw\/web\/csh\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=21614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}